NB-4 Cells

Name: CLS Cell Lines Service GmbH
Address: Dr.-Eckener-Straße 8, Eppelheim, 69214, DE
Telephone: +496221405780
Price range: €€€

CAD$545.10*

Products are shipped frozen on dry ice in cryotubes. Each cryotube typically contains 3 × 10⁶ cells for adherent lines or 5 × 10⁶ cells for suspension lines (refer to the batch CoA for details).

Prices excl. taxes plus shipping costs

cryovial

Product number: 300299

Safety data sheet

Product sheet

Certificate of Analysis (CoA)

Description	NB-4 cells are a human acute promyelocytic leukemia (APL) cell line established from the bone marrow of a patient experiencing the second relapse of acute promyelocytic leukemia. This cell line is characterized by the presence of the t(15;17) chromosomal translocation, which results in the PML-RARα fusion gene, a hallmark of APL. The NB4 cell line serves as a pivotal model for studying the pathogenesis of APL and the mechanisms of action of therapeutic agents inducing differentiation such as retinoic acid (ATRA) and arsenic trioxide (ATO). As a promyelocytic leukemia cell line, NB-4 cells exhibit an aberrant pattern of differentiation that is characteristic of APL. This aberrancy provides a unique window into the cellular mechanisms underlying leukemia progression and the potential for therapeutic intervention. The ability of NB-4 cells to undergo apoptosis, or programmed cell death, upon exposure to certain chemotherapeutic agents or differentiation inducers like retinoic acid, makes them an invaluable tool for studying cell apoptosis in the context of leukemia. The NB-4 cell line also demonstrates bilineage potential, highlighting its ability to differentiate along multiple hematopoietic lineages under specific conditions. In conclusion, the NB-4 cell line, with its unique properties and responsiveness to differentiation inducers like retinoic acid, continues to be a pivotal resource for researchers delving into the intricacies of promyelocytic leukemia and the broader field of oncology.
Organism	Human
Tissue	Bone marrow
Disease	Acute promyelocytic leukemia
Synonyms	NB4, NB.4

Age	23 years
Gender	Female
Ethnicity	Caucasian
Morphology	Round cells
Cell type	B lymphocyte
Growth properties	Suspension

Citation	NB-4 (Cytion catalog number 300299)
Biosafety level	1
NCBI_TaxID	9606
CellosaurusAccession	CVCL_0005

Antigen expression	CD4+, CD14-, CD36-
Reverse transcriptase	Negative
Karyotype	T(15,17) (q22,q11-12) translocation

Culture Medium	RPMI 1640, w: 2.0 mM stable Glutamine, w: 2.0 g/L NaHCO3 (Cytion article number 820700a)
Supplements	Supplement the medium with 10% FBS
Doubling time	35 to 40 hours
Subculturing	Maintain cultures by periodically adding or replacing the medium. Initiate cultures with a density of 5 x 10⁵ cells/ml and keep the cell concentration within the range of 3 x 10⁵ to 1 x 10⁶ cells/ml for optimal growth.
Freeze medium	As a cryopreservation medium, we use complete growth medium (including FBS) + 10% DMSO for adequate post-thaw viability, or CM-1 (Cytion catalog number 800100), which includes optimized osmoprotectants and metabolic stabilizers to enhance recovery and reduce cryo-induced stress.
Thawing and Culturing Cells	Confirm that the vial remains deeply frozen upon delivery, as cells are shipped on dry ice to maintain optimal temperatures during transit. Upon receipt, either store the cryovial immediately at temperatures below -150°C to ensure the preservation of cellular integrity, or proceed to step 3 if immediate culturing is required. For immediate culturing, swiftly thaw the vial by immersing it in a 37°C water bath with clean water and an antimicrobial agent, agitating gently for 40-60 seconds until a small ice clump remains. Perform all subsequent steps under sterile conditions in a flow hood, disinfecting the cryovial with 70% ethanol before opening. Carefully open the disinfected vial and transfer the cell suspension into a 15 ml centrifuge tube containing 8 ml of room-temperature culture medium, mixing gently. Centrifuge the mixture at 300 x g for 3 minutes to separate the cells and carefully discard the supernatant containing residual freezing medium. Gently resuspend the cell pellet in 10 ml of fresh culture medium. For adherent cells, divide the suspension between two T25 culture flasks; for suspension cultures, transfer all the medium into one T25 flask to promote effective cell interaction and growth. Adhere to established subculture protocols for continued growth and maintenance of the cell line, ensuring reliable experimental outcomes.
Incubation Atmosphere	37°C, 5% CO₂, humidified atmosphere.
Shipping Conditions	Cryopreserved cell lines are shipped on dry ice in validated, insulated packaging with sufficient refrigerant to maintain approximately −78 °C throughout transit. On receipt, inspect the container immediately and transfer vials without delay to appropriate storage.
Storage Conditions	For long-term preservation, place vials in vapor-phase liquid nitrogen at about −150 to −196 °C. Storage at −80 °C is acceptable only as a short interim step before transfer to liquid nitrogen.

Sterility	Mycoplasma contamination is excluded using both PCR-based assays and luminescence-based mycoplasma detection methods. To ensure there is no bacterial, fungal, or yeast contamination, cell cultures are subjected to daily visual inspections.

Lot Number	Certificate Type	Date	Catalog Number
300299-110725	Certificate of Analysis	18. Aug. 2025	300299
300299-060923	Certificate of Analysis	23. May. 2025	300299

Required products

Required products

Antibiotic/Antimycotic Solution (100x)

Product Overview
Volume: 100 ml
Storage: ≤-15°C
Sterility: Sterile-filtered
Antibiotic/Antimycotic Solution (100x) is a sterile, ready-to-use concentrate designed to reduce microbial contamination risks in cell culture and related laboratory applications. This 100x solution contains a well-established combination of penicillin, streptomycin, and amphotericin B—providing broad-spectrum antimicrobial activity against Gram-positive and Gram-negative bacteria, yeasts, and filamentous fungi. The formulation is suitable for use in eukaryotic cell cultures, bacterial media, and other contamination-sensitive systems, supporting clean and consistent lab operations.
Application and Benefits Optimized for routine research protocols, this solution is widely used to maintain aseptic conditions in cell culture workflows. It offers reliable performance in contamination-sensitive environments, helping researchers reduce the risk of microbial overgrowth without compromising cell health or experimental reproducibility. The sterile-filtered formulation eliminates the need for additional solubilization steps, supporting streamlined media preparation and reducing variability in daily lab procedures.
Usage and Compatibility To achieve standard working concentrations, dilute the solution 1:100 into your complete culture medium. The product is compatible with a broad range of mammalian cell lines and basal media. With consistent stock availability, researchers benefit from dependable supply continuity and simplified logistics planning. The solution should be stored at ≤ –15 °C and protected from repeated freeze-thaw cycles to maintain stability. For research use only. Not for use in diagnostic or therapeutic procedures. Not for use in humans or animals.

CAD$62.10*

NB-4 Cells

Introduction to the NB-4 cell line

Characteristics

Identifiers / Biosafety / Citation

Genetic makeup of the promyelocytic leukemia NB4 cell line

Culturing guidelines

Quality assurance of NB4 cells

Certificate of Analysis (CoA)