A549-RFP Cells

Name: CLS Cell Lines Service GmbH
Address: Dr.-Eckener-Straße 8, Eppelheim, 69214, DE
Telephone: +496221405780
Price range: €€€

USD$800.00*

Products are shipped frozen on dry ice in cryotubes. Each cryotube typically contains 3 × 10⁶ cells for adherent lines or 5 × 10⁶ cells for suspension lines (refer to the batch CoA for details).

Prices excl. taxes plus shipping costs

cryovial

Product number: 305659

Safety data sheet

Product sheet

Description	A549-RFP is a fluorescently labeled derivative of the human A549 lung adenocarcinoma cell line, engineered to constitutively express red fluorescent protein (RFP) for real-time visualization and tracking. The parental A549 line was established from a lung adenocarcinoma derived from an adult donor and exhibits epithelial morphology with adherent growth characteristics. A549 cells retain features of type II alveolar epithelial cells, including expression of cytokeratins and the capacity for surfactant-associated protein production. The introduction of a stable RFP expression cassette enables continuous fluorescence without significantly altering the intrinsic proliferative and metabolic properties of the parental line, making A549-RFP suitable for longitudinal imaging studies. Functional characterization of A549 cells within large cancer cell panels has demonstrated that cell size, protein content, and protein synthesis rate are positively correlated with cell volume, and that larger cells tend to proliferate more slowly. In comparative analyses, A549 cells are positioned among relatively smaller, faster-proliferating epithelial cancer cell lines, in contrast to larger, more mesenchymal-like cells that display higher vimentin expression and lower E-cadherin levels. These metabolic and phenotypic distinctions are relevant for experimental interpretation, as protein synthesis rates and metabolic fluxes scale with cell size and influence sensitivity to agents targeting proliferation or mTOR-regulated anabolic pathways. The RFP modification preserves the suitability of A549 cells for such metabolic and pharmacologic investigations while enabling direct visualization. A549-RFP is widely used in co-culture systems, orthotopic and ectopic xenograft models, and invasion or metastasis assays where fluorescent labeling facilitates discrimination of tumor cells from stromal or host components. The stable red fluorescence supports applications including live-cell imaging, high-content screening, flow cytometry-based quantification, and in vivo optical imaging. As a traceable variant of a well-characterized lung adenocarcinoma model, A549-RFP provides a robust platform for studying tumor cell proliferation, epithelial-mesenchymal transition, drug response, and tumor-microenvironment interactions in both in vitro and in vivo settings.
Organism	Human
Tissue	Lung
Disease	Lung adenocarcinoma
Synonyms	A 549, A549, NCI-A549, A549/ATCC, A549 ATCC, A549ATCC, hA549

Age	58 years
Gender	Male
Ethnicity	Caucasian

Citation	A549-RFP (Cytion catalog number 305659)
Biosafety level	1
NCBI_TaxID	9606
CellosaurusAccession	CVCL_0023
GMO Status	GMO-S1: This A549 lung carcinoma line contains a lentiviral RFP construct enabling red fluorescence imaging. This classification applies only within Germany and may differ elsewhere.

Protein expression	RFP
Mutational profile	Mutation: p.Gly12Ser, Homozygous; Mutation: p.Gln37Ter, Homozygous

Culture Medium	DMEM, w: 4.5 g/L Glucose, w: 4 mM L-Glutamine, w: 3.7 g/L NaHCO3, w: 1.0 mM Sodium pyruvate (Cytion article number 820300a)
Supplements	Supplement the medium with 10% FBS
Dissociation Reagent	Accutase
Doubling time	20-40 hours
Freeze medium	As a cryopreservation medium, we use complete growth medium + 10% DMSO for adequate post-thaw viability.
Thawing and Culturing Cells	Confirm that the vial remains deeply frozen upon delivery, as cells are shipped on dry ice to maintain optimal temperatures during transit. Upon receipt, either store the cryovial immediately at temperatures below -150°C to ensure the preservation of cellular integrity, or proceed to step 3 if immediate culturing is required. For immediate culturing, swiftly thaw the vial by immersing it in a 37°C water bath with clean water and an antimicrobial agent, agitating gently for 40-60 seconds until a small ice clump remains. Perform all subsequent steps under sterile conditions in a flow hood, disinfecting the cryovial with 70% ethanol before opening. Carefully open the disinfected vial and transfer the cell suspension into a 15 ml centrifuge tube containing 8 ml of room-temperature culture medium, mixing gently. Centrifuge the mixture at 200 x g for 5 minutes, carefully discard the supernatant containing freezing medium. Follow the procedure described under Post-Thaw Recovery
Incubation Atmosphere	37°C, 5% CO₂, humidified atmosphere.
Shipping Conditions	Cryopreserved cell lines are shipped on dry ice in validated, insulated packaging with sufficient refrigerant to maintain approximately −78 °C throughout transit. On receipt, inspect the container immediately and transfer vials without delay to appropriate storage.
Storage Conditions	For long-term preservation, place vials in vapor-phase liquid nitrogen at about −150 to −196 °C. Storage at −80 °C is acceptable only as a short interim step before transfer to liquid nitrogen.

Required products

Required products

Antibiotic/Antimycotic Solution (100x)

Product Overview
Volume: 100 ml
Storage: ≤-15°C
Sterility: Sterile-filtered
Antibiotic/Antimycotic Solution (100x) is a sterile, ready-to-use concentrate designed to reduce microbial contamination risks in cell culture and related laboratory applications. This 100x solution contains a well-established combination of penicillin, streptomycin, and amphotericin B—providing broad-spectrum antimicrobial activity against Gram-positive and Gram-negative bacteria, yeasts, and filamentous fungi. The formulation is suitable for use in eukaryotic cell cultures, bacterial media, and other contamination-sensitive systems, supporting clean and consistent lab operations.
Application and Benefits Optimized for routine research protocols, this solution is widely used to maintain aseptic conditions in cell culture workflows. It offers reliable performance in contamination-sensitive environments, helping researchers reduce the risk of microbial overgrowth without compromising cell health or experimental reproducibility. The sterile-filtered formulation eliminates the need for additional solubilization steps, supporting streamlined media preparation and reducing variability in daily lab procedures.
Usage and Compatibility To achieve standard working concentrations, dilute the solution 1:100 into your complete culture medium. The product is compatible with a broad range of mammalian cell lines and basal media. With consistent stock availability, researchers benefit from dependable supply continuity and simplified logistics planning. The solution should be stored at ≤ –15 °C and protected from repeated freeze-thaw cycles to maintain stability. For research use only. Not for use in diagnostic or therapeutic procedures. Not for use in humans or animals.

USD$45.00*

A549-RFP Cells

General information

Characteristics

Regulatory Data

Biomolecular Data

Handling

Quality Control & Molecular Analysis