SiHa Cells

Name: CLS Cell Lines Service GmbH
Address: Dr.-Eckener-Straße 8, Eppelheim, 69214, DE
Telephone: +496221405780
Price range: €€€

USD$395.00*

Products are shipped frozen on dry ice in cryotubes. Each cryotube typically contains 3 × 10⁶ cells for adherent lines or 5 × 10⁶ cells for suspension lines (refer to the batch CoA for details).

Prices excl. taxes plus shipping costs

cryovial

Product number: 305023

Safety data sheet

Product sheet

Certificate of Analysis (CoA)

Description	SiHa cells are a human cervical squamous cell carcinoma cell line that has been widely used in research for several decades. They were isolated from primary uterine biopsy fragments from a 55-year-old female Japanese patient with squamous cell carcinoma. This cell line is of great interest to researchers studying cervical cancer and other related diseases due to their unique genetic characteristics. SiHa cells have been found to express the p53+ and pRB+ genes, which are involved in cell cycle regulation, DNA repair, and tumor suppression. These genes make SiHa cells an ideal model for studying the molecular mechanisms of cancer development and progression. Additionally, SiHa cells are a suitable transfection host, making them an excellent tool for gene expression studies. SiHa cells have a hypertriploid karyotype, with an average chromosome number between 69 and 72. The SiHa cells are HPV-16 positive, showing integration of 1 to 2 copies of the viral genome per cell. Cells are tumorigenic, forming poorly differentiated epidermoid carcinoma (grade III) in nude mice. This makes them an excellent model for studying cancer progression and testing anti-cancer drugs. SiHa cell line expresses various isoenzymes, including AK-1, ES-D, G6PD, GLO-I, Me-2, PGM1, and PGM3. Electron microscopy revealed abundant tonofilaments in the cytoplasm and desmosomes at the cell junctions. The growth properties of SiHa cells are adherent, with a doubling time of 17 hours in 10% FBS media and 21 hours in 5% FBS media. Epithelial cell adhesion molecule (EpCAM) expression is present in 92% of SiHa cells, indicating their epithelial origin. They show strong cytokeratin expression but no vimentin expression.
Organism	Human
Tissue	Cervix
Disease	Human papillomavirus-related cervical squamous cell carcinoma
Synonyms	Siha, SIHA

Age	55 years
Gender	Female
Ethnicity	Asian
Morphology	Epithelial
Growth properties	Adherent

Citation	SiHa (Cytion catalog number 305023)
Biosafety level	1
NCBI_TaxID	9606
CellosaurusAccession	CVCL_0032

Tumorigenic	Yes

Culture Medium	EMEM (MEM Eagle), w: 2 mM L-Glutamine, w: 2.2 g/L NaHCO3, w: EBSS (Cytion article number 820100a)
Supplements	Supplement the medium with 10% FBS and 1% NEAA
Dissociation Reagent	Accutase
Subculturing	Remove the old medium from the adherent cells and wash them with PBS that lacks calcium and magnesium. For T25 flasks, use 3-5 ml of PBS, and for T75 flasks, use 5-10 ml. Then, cover the cells completely with Accutase, using 1-2 ml for T25 flasks and 2.5 ml for T75 flasks. Let the cells incubate at room temperature for 8-10 minutes to detach them. After incubation, gently mix the cells with 10 ml of medium to resuspend them, then centrifuge at 300xg for 3 minutes. Discard the supernatant, resuspend the cells in fresh medium, and transfer them into new flasks that already contain fresh medium.
Fluid renewal	2 to 3 times per week
Freeze medium	As a cryopreservation medium, we use complete growth medium (including FBS) + 10% DMSO for adequate post-thaw viability, or CM-1 (Cytion catalog number 800100), which includes optimized osmoprotectants and metabolic stabilizers to enhance recovery and reduce cryo-induced stress.
Thawing and Culturing Cells	Confirm that the vial remains deeply frozen upon delivery, as cells are shipped on dry ice to maintain optimal temperatures during transit. Upon receipt, either store the cryovial immediately at temperatures below -150°C to ensure the preservation of cellular integrity, or proceed to step 3 if immediate culturing is required. For immediate culturing, swiftly thaw the vial by immersing it in a 37°C water bath with clean water and an antimicrobial agent, agitating gently for 40-60 seconds until a small ice clump remains. Perform all subsequent steps under sterile conditions in a flow hood, disinfecting the cryovial with 70% ethanol before opening. Carefully open the disinfected vial and transfer the cell suspension into a 15 ml centrifuge tube containing 8 ml of room-temperature culture medium, mixing gently. Centrifuge the mixture at 300 x g for 3 minutes to separate the cells and carefully discard the supernatant containing residual freezing medium. Gently resuspend the cell pellet in 10 ml of fresh culture medium. For adherent cells, divide the suspension between two T25 culture flasks; for suspension cultures, transfer all the medium into one T25 flask to promote effective cell interaction and growth. Adhere to established subculture protocols for continued growth and maintenance of the cell line, ensuring reliable experimental outcomes.
Incubation Atmosphere	37°C, 5% CO₂, humidified atmosphere.
Shipping Conditions	Cryopreserved cell lines are shipped on dry ice in validated, insulated packaging with sufficient refrigerant to maintain approximately −78 °C throughout transit. On receipt, inspect the container immediately and transfer vials without delay to appropriate storage.
Storage Conditions	For long-term preservation, place vials in vapor-phase liquid nitrogen at about −150 to −196 °C. Storage at −80 °C is acceptable only as a short interim step before transfer to liquid nitrogen.

Sterility	Mycoplasma contamination is excluded using both PCR-based assays and luminescence-based mycoplasma detection methods. To ensure there is no bacterial, fungal, or yeast contamination, cell cultures are subjected to daily visual inspections.

Lot Number	Certificate Type	Date	Catalog Number
305023-190825	Certificate of Analysis	22. Oct. 2025	305023
305023-160124	Certificate of Analysis	23. May. 2025	305023

Required products

Required products

Antibiotic/Antimycotic Solution (100x)

Product Overview
Volume: 100 ml
Storage: ≤-15°C
Sterility: Sterile-filtered
Antibiotic/Antimycotic Solution (100x) is a sterile, ready-to-use concentrate designed to reduce microbial contamination risks in cell culture and related laboratory applications. This 100x solution contains a well-established combination of penicillin, streptomycin, and amphotericin B—providing broad-spectrum antimicrobial activity against Gram-positive and Gram-negative bacteria, yeasts, and filamentous fungi. The formulation is suitable for use in eukaryotic cell cultures, bacterial media, and other contamination-sensitive systems, supporting clean and consistent lab operations.
Application and Benefits Optimized for routine research protocols, this solution is widely used to maintain aseptic conditions in cell culture workflows. It offers reliable performance in contamination-sensitive environments, helping researchers reduce the risk of microbial overgrowth without compromising cell health or experimental reproducibility. The sterile-filtered formulation eliminates the need for additional solubilization steps, supporting streamlined media preparation and reducing variability in daily lab procedures.
Usage and Compatibility To achieve standard working concentrations, dilute the solution 1:100 into your complete culture medium. The product is compatible with a broad range of mammalian cell lines and basal media. With consistent stock availability, researchers benefit from dependable supply continuity and simplified logistics planning. The solution should be stored at ≤ –15 °C and protected from repeated freeze-thaw cycles to maintain stability. For research use only. Not for use in diagnostic or therapeutic procedures. Not for use in humans or animals.

USD$45.00*

SiHa Cells

General information

Characteristics

Regulatory Data

Biomolecular Data

Handling

Quality Control & Molecular Analysis

Certificate of Analysis (CoA)